Fig. 2: Physical characterization of Knock-beads.
From: Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening
a The chemical synthesis route of Knock-beads. Created in BioRender. Guo, F. (2024) BioRender.com/y59k149. b SEM images showing the core part of Knock-beads at different scales: Fe3O4-Au (top, scale bar 70 μm), Fe3O4 beads (middle, scale bar 5 μm), and Fe3O4-Au (bottom, scale bar 2 μm). c Fluorescence image showing Fe3O4 magnetic beads that were first labeled with Alexa-647 dye and then conjugated to Au nanorods. Created in BioRender. Guo, F. (2023) BioRender.com/d92g406. d Fluorescence image showing the Au nanorods that were first labeled with Alexa-647 dye and then conjugated to Fe3O4 magnetic beads. Created in BioRender. Guo, F. (2023) BioRender.com/d92g406. e Dynamic light scattering results showing the size variation of Fe3O4, Fe3O4-Au, Fe3O4-Au-PDDA beads and Fe3O4-Au-PDDA-siRNA conjugates (8 × 105 beads, 20 pmol siRNA input). f Zeta potential analysis of Fe3O4, Fe3O4-Au, Fe3O4-Au-PDDA beads and Fe3O4-Au-PDDA-siRNA conjugates (8 × 105 beads, 20 pmol siRNA input). g Fluorescent image of Knock-beads in PBS solution, scale bar 20 μm. Three independent experiments were conducted (b, c, d, g). Source data are provided as a Source Data file.
