Fig. 5: Combinatorial screening of siRNA cocktails for anti-tumor efficacy.
From: Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening
a Six siRNAs that related to cell apoptosis (survivin, Bcl-2, Mcl-1) and cell cycle (c-Myc, Cyclin D1, PLK-1) pathway form 64 combinations for relevant gene knock down. Created in BioRender. Guo, F. (2024) BioRender.com/v53d647. b Combined fluorescence image showing 7 types of spectrum-coded (Alexa-514, Alexa-568, and Alexa-647) Knock-beads. Confocal microscopic images showing the staining of apoptosis (c, TUNEL) or proliferation (d, Edu) markers for gene-silencing phenotype in cells treated with scCode-fection. The Knock-beads were overlayed with the cells. c Fluorescence image of actin (green) and TUNEL (magenta) staining to show cell apoptosis level. d Fluorescence image of Edu (cyan) staining to show cell proliferation level. The cell outlines (white) were derived from bright-field images. e Distribution of the number of Knock-beads on individual cells. f Combinatorial screening of 54 Knock-combos composed of 6 siRNAs for anti-tumor efficacy. The siRNA combinations are marked in the gray-shaded table (middle). The bar charts show the induction of cellular apoptosis (left) or inhibition of cell proliferation (right) as the result of gene-silencing by correspondent siRNA Knock-combos. Three rounds of screening were performed. For each knock combo, n > 20 per round, *P < 0.05, **P < 0.01, ***P < 0.001, analyzed using unpaired one-way ANOVA. More details about the statistical significance are provided in Supplementary Table 7. For panel (b–e), three independent experiments were conducted. Source data are provided as a Source Data file.
