Fig. 7: Expanded screening of 1315 Knock-combos composed of 1, 2, or 3 siRNAs targeting 20 genes.
From: Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening
a A set of 20 siRNAs targeting 6 different cellular pathways: membrane interaction (5 siRNAs), apoptosis (4 siRNAs), cell cycle (2 siRNAs), DNA repair (3 siRNAs), drug resistance (1 siRNA), and oncogenesis (5 siRNAs). The screening space (1350) is composed of 20 1-siRNA, 190 2-siRNA and 1140 3-siRNA Knock-combos. b Phenotypic analysis of apoptosis induction for all the tested 1-siRNA (blue), 2-siRNA (red) and 3-siRNA (yellow) Knock-combos. Data were collected from more than 20 cells for each of the 1315 Knock-combos. c Boxplots with scattered data points showing the comparison of the efficacy on apoptosis induction for combinatorial targeting of single, dual or multiple signaling pathways. d K-means clustering analysis of all the Knock-combos reveals two distinct clusters in the UMAP space. e Boxplots showing the comparison of apoptosis induction for the 2 identified Knock-combo clusters. f Enrichment analysis of the 20 siRNA targets in each identified Knock-combo clusters. g An interaction network showing the synergic co-operation in apoptosis induction for the 20 siRNAs to different gene targets, where the line color and thickness indicate the phenotypic efficacy of the corresponding combination. For panel (c, e), n = 7 for each Knock-combo, the data was analyzed using non-parametric Mann–Whitney test. The black dots indicate an overlay of individual data points, each box indicates the first quartile, median, third quartile of the data, the whisker limit is 1.5 times the interquartile range. Source data are provided as a Source Data file.
