Fig. 3: Either Met4 or Met28 can sustain C. parapsilosis growth in the absence of methionine.
From: Alternative sulphur metabolism in the fungal pathogen Candida parapsilosis
A C. parapsilosis CLIB214 and derivative strains disrupted in MET4, MET28, or both were tested for their ability to grow in the absence of sulphur-containing amino acids. Cell dilutions were spotted on SC media supplemented with all amino acids (SC + AA), or lacking cysteine/methionine (SC – C/M), cysteine (SC – C) or methionine (SC – M) and photographed after 48 h at 30 °C. Only disrupting both MET4 and MET28, either by deletion or by insertion of a premature stop codon (met4Δ/met28Δ and Ed met4/met28), abolished methionine synthesis. Restoration of the wildtype sequence of either MET4 (cMET4) or MET28 (cMET28) recovered methionine prototrophy. B The same result was obtained when the mutations were introduced in three additional C. parapsilosis strains from Clades 5 (02−203), 3 (81-042), and 4 (CDC179)58. On the contrary, disruption of MET28 in the related species C. tropicalis (CAS08-102) was enough to abolish methionine synthesis. Source data (uncropped photographs) are provided as a Source Data file.
