Fig. 6: C. parapsilosis sulphur responsive genes contain Cbf1 and Met32 binding sites.

The promoter regions (1-kb regions upstream of the starting codon) of the genes upregulated in the absence of Met/Cys were analysed using MEME-suite⁶³. The promoters are depicted as lines (0 = −1000bp from ATG; 1000 = − 1bp from ATG). The genes are listed on the left, colour-coded based on their dependency on Met4 (pink) or Met28 (light blue). Two motifs were found, corresponding to sites that are known to mediate the DNA binding of Met32 and Cbf1 in S. cerevisiae (illustrated above the graph). The distribution of the motifs is indicated by red (Met32) or blue (Cbf1) dots, located above (+ strand) or below (− strand) the promoter lines. The precise coordinates are provided in Supplementary Table 3. The promoters contain Met32 only, Cbf1 only, or both binding sites (indicated by the motif checkboard on the left-hand side; grey box: no motifs found with the selected stringency on MEME).