Fig. 4: IncA clustering in ICSs requires the presence of IncA on both membranes.
From: Specialized contact sites regulate the fusion of chlamydial inclusion membranes
a If IncA interacts in trans, then clustering will only be observed when IncA is present on both inclusion membranes. WT = wild-type. KO = IncA knockout. Created in BioRender. Paumet, F. (2024) BioRender.com/w90v943. b If IncA interacts in cis, then IncA clustering will be observed when IncA is on one or both inclusion membranes. Created in BioRender. Paumet, F. (2024) BioRender.com/q49y738. c, d HeLa cells were co-infected with IncAKO Ctr (MOI 2.5) and IncAchimera-FLAG Ctr (MOI 2.5) (c), or with IncAKO Ctr (MOI 2.5) and WT Ctr (MOI 2.5) (d) in the presence of anhydrotetracycline. Cells were fixed at 24 hpi and labeled with anti-MOMP (magenta), which labels Ctr, anti-IncA (green), and anti-IPAM (red) antibodies. DNA was stained with Hoechst (blue). For c, d: Asterisks denote inclusions; Scale bars = 10 μm. Images are representative of three independent experiments. e ES quantification for the co-infections conducted in c and d. Graph depicts the average (black line) and individual ESs (dots). n = 150 total scores per Inc from three independent experiments. Gray box denotes ESs <2. ns = not significant, * denotes p-value < 0.05, *** denotes p-value < 0.001 (two-way ANOVA).
