Fig. 3: In vitro experimental validation of a CHN encoded text file using nanopore sequencing.

a The time consumption for each step in a serial scheme. b An example of raw data from nanopore sequencing, the corresponding alphabet sequence after base calling and decoded digital sequence. c Effective nanopore reads per composite strand as a function of the sequencing time. Violin plots with embedded box-and-whiskers show the distribution of variant composite strands (n = 122). The gray dot represents the median, and the thick center line shows the quartiles. d The reconstructed text file at the time stamps of 0–2, 4, 6–9, 10, and 20 min, respectively. e The top absolute coefficient of variance for each composite strand and the bottom relative coefficient of variance as a function of time. The bottom violin plots with embedded box-and-whiskers show the distribution of variant text composite strands (n = 7). The gray dot represents the median, and the thick center line shows the quartiles. f The binary data recovery as a function of sequencing time. Source data are provided as a Source Data file.