Fig. 1: Design of chiRyR. | Nature Communications

Fig. 1: Design of chiRyR.

From: Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance

Fig. 1

a Chemical structures of FLU, TET, and CHL. b Dose-dependent effects of FLU (top) and TET (bottom) on F/F0 in cell lines expressing rRyR1, Sf RyR, or chiRyR (n = 3). The average fluorescence intensity of the last 100 seconds (F) was normalized to that of the initial 100 seconds (F0) to reflect the luminal Ca2+ concentration change in the ER. The data are shown as the mean values ± SD. Source data are provided as a Source Data file. c Sequence alignment of diamide insecticide-binding sequences in RyRs. The four non-conserved residues involved in diamide coordination, which are selected to generate chiRyR, are colored in purple, and the other diamide-coordinating residues are colored in blue. d Comparison of the diamide-binding pocket in rRyR1 (white, PDB ID: 6M2W) and Sf RyR (orange, homology model based on 6M2W). The insect-specific diamide-coordinating residues are colored in purple.

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