Fig. 4: Mutations in RyR cause resistance to diamide insecticides. | Nature Communications

Fig. 4: Mutations in RyR cause resistance to diamide insecticides.

From: Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance

Fig. 4

a Locations of the resistance mutations in RyR. Mutation sites, colored in black, cluster near the diamide binding site. b, c Double resistance mutations reduce the sensitivity of chiRyR to FLU (left) and CHL (right) as shown by time-lapse [Ca2+]ER measurements (b) and [3H]ryanodine binding assays (c). The data are shown as the mean values ± SD (n = 3 for time-lapse; n = 4 for [3H]ryanodine binding). Source data are provided as a Source Data file. d FLU/CHL toxicity curves for gene-edited Drosophila (n = 30) expressing RyR M4758I (corresponding to Sf RyR WT) or RyR G4915E (corresponding to Sf RyR-I4734M/G4891E). Mortality rates were calculated after 24 h of feeding with either FLU or CHL. The data are shown as the mean values ± SD. Source data are provided as a Source Data file.While the resistance mutations reduce the potency of both insecticides, CHL is relatively more potent compared to FLU against the resistant Drosophila.

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