Fig. 4: Differential contributions of newly fused and pre-existing myonuclei to adult muscle adaptations. | Nature Communications

Fig. 4: Differential contributions of newly fused and pre-existing myonuclei to adult muscle adaptations.

From: Lineage tracing of nuclei in skeletal myofibers uncovers distinct transcripts and interplay between myonuclear populations

Fig. 4

a Schematic diagram of the single-nuclei RNA-seq methodology applied to HSArtTA; TREH2B-GFP mice, where all myonuclei were labeled with GFP and sorted for subsequent sequencing analysis. b Integrated UMAP from plantaris muscle following overload stimulation (n = 2) or under sham condition (n = 1) (HSArtTA; TREH2B-GFP treated with Dox as depicted in (a)). c Split UMAP view of myogenic nuclei shown in (b). d Proportion of unclassified A, B, and Atf3+ nuclear populations. e Dot plot depicts selected genes differentially expressed in various myonuclear populations. Dot size represents the percentage of nuclei expressing the gene. Source data are provided as a Source Data file.

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