Fig. 5: smRNA-FISH analyses validates gene expression in newly fused and pre-existing myonuclei. | Nature Communications

Fig. 5: smRNA-FISH analyses validates gene expression in newly fused and pre-existing myonuclei.

From: Lineage tracing of nuclei in skeletal myofibers uncovers distinct transcripts and interplay between myonuclear populations

Fig. 5

a Representative images of smRNA-FISH on sections from Pax7rtTA; TREH2B-GFP muscle for selected genes enriched in newly fused (H19, Igf2, Tnnt1, Tnnt2) or pre-existing myonuclei (Atf3). Quantification of the signal for each gene is shown on the right. (n = 3–5 independent animals). Scale bar, 20 μm. b Representative images of smRNA-FISH for the indicated genes on sections from control (MymkloxP/loxp + tamoxifen) or MymkscKO mice subjected to muscle overload. Quantification of the signals are shown on the bottom. (n = 3–6 independent animals). Scale bar, 20 μm. c Representative images of smRNA-FISH for Myh3, Tnnt1, and Tnnt2 on sections from Pax7rtTA; TREH2B-GFP muscle during postnatal development. (n = 3). Scale bar, 20 μm. Data are presented as mean ± SD. Statistical tests used were (a) one-way ANOVA with Tukey’s correction for multiple comparisons; (b) two-tailed unpaired t-test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.

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