Fig. 6: Comparative gene features of newly fused myonuclei.

a Representative images of smRNA-FISH for Myh3 on plantaris myofibers from Pax7^rtTA; TRE^H2B-GFP mice undergoing overload stimulation or under sham conditions. Myh3 transcription was detected in both peripheral (white arrow) and central (arrowhead) newly fused myonuclei. (n = 3–4). Scale bar, 20 μm. b Representative images of smRNA-FISH for Myh3, performed on the same mice used in (A), show that both central newly fused GFP⁺ myonuclei (arrowhead) and peripheral newly fused GFP⁺ (white arrow) are enriched for Myh3 transcription. (n = 3–4). Scale bar, 20 μm. c Heatmap of genes showing distinct transcriptional signature in nuclei of myogenic populations from plantaris muscle subjected to 1 week of overload stimulation, as used in Fig. 4c (n = 2).