Fig. 2: Tubular ER is associated with active myelination.
From: Nonvesicular lipid transfer drives myelin growth in the central nervous system
a, b Immunohistochemistry of P14 mouse cortex. Arrow heads: pre-myelinating oligodendrocytes (MAG-BCAS1+); arrows: myelinating oligodendrocytes (MAG+BCAS1+). c Quantification of (a) and (b) showing mean \(\pm \,\)SD. 1.65\(\pm\)0.55% or 3.54\(\pm\)2.60% premyelinating cells are REEP5+ or RTN4+, 93.04\(\pm\)0.25% or 82.34\(\pm\)4.16% myelinating cells are REEP5+ or RTN4+ (n = 3 wild-type P14 mice, two-way ANOVA followed by Sidak’s multiple comparison test, ****P < 0.0001). d, f Immunohistochemistry of P14 and six-month-old (6mo) adult mouse thoracic spinal cord, focusing on dorsal white matter e, g quantification of tubular ER (REEP5 or RTN4) fluorescence signal normalized by myelin (MBP) signal, in the dorsal white matter of thoracic spinal cord, showing mean \(\pm \,\)SD, (e) P14: 0.95\(\pm\)0.27, 6-month-old: 0.27\(\pm\)0.06 (n = 3 wildtype mice for each time point, two-tailed unpaired t-test, t = 4.302, df=4, *p = 0.0126) (g) P14: 1.35\(\pm\)0.30, 6-month-old: 0.46\(\pm\)0.13 (n = 3 wildtype mice for each time point, two-tailed unpaired t-test, t = 4.677, df=4, **p = 0.0095) h Left: Immunocytochemistry of primary oligodendrocyte culture for tubular ER markers (RTN4 and RTN1), rough ER marker SEC61B, ER sheet marker KDEL40 and oligodendrocyte marker O1 and MBP for outlining the cells. Right: The ratio of fluorescence signals from different ER subtypes at the cellular processes to that at the cell body, showing mean ± SD, KDEL: 0.58\(\pm\)0.10, RTN1: 0.79\(\pm\)0.11, SEC61B: 0.56\(\pm\)0.10, RTN4: 0.75\(\pm\)0.09 (n = 24 cells for KDEL/RTN1, 25 cells for SEC61B/RTN4, one-way ANOVA, F (3, 94) = 36.09, Tukey’s post-hoc test: **** p < 0.0001) Scale bars: 50 µm (a, b), 100 µm (d, f), 10 µm (h). Source data are provided as a Source Data file.
