Fig. 3: Glycolipid transfer protein (GLTP) is associated with active myelination.
From: Nonvesicular lipid transfer drives myelin growth in the central nervous system
a Immunohistochemistry of P14 mouse cortex. Arrow heads: pre-myelinating oligodendrocytes (MAG-BCAS1+); arrows: myelinating oligodendrocytes (MAG+BCAS1+). b Quantification of (a) showing mean \(\pm \,\)SD, 3.33\(\pm\)1.36% premyelinating cells are GLTP+, 92.62\(\pm\)3.08% myelinating cells are GLTP+ (n = 3 wild-type P14 mice, two-tailed paired t-test, t = 87.45, df=2, ***p = 0.0001) c Immunohistochemistry of P14 and six-month-old (6mo) adult mouse thoracic spinal cord, focusing on dorsal white matter. d quantification of GLTP fluorescence signal normalized by myelin (MBP) signal, in dorsal white matter of thoracic spinal cord, showing mean \(\pm \,\)SD, P14: 0.76\(\pm\)0.19, 6-month-old: 0.39\(\pm\)0.03 (n = 3 wildtype mice for each time point, Two-tailed unpaired t-test, t = 3.403, df=4, *p = 0.0272). e zoom-in view of P14 spinal cord f Ratio of myelin (MBP+ ring) overlapping with GLTP. Quantification of (e) showing mean \(\pm \,\)SD, 99.51\(\pm\)0.44% myelin overlaps with GLTP (n = 3 wild-type P14 mice). g Immunocytochemistry of primary oligodendrocyte culture showing GLTP subcellular localization. h The ratio of fluorescence signals from GLTP and KDEL at the cellular processes to that at the cell body, showing mean ± SD, GLTP: 0.54 ± 0.20, KDEL: 0.32 ± 0.09 (n = 24 cells, two-tailed paired t-test, t = 5.834, df=23, ****p < 0.0001). Scale bars: 50 µm (a), 100 µm (c), 1 µm (e), 10 µm (g). Source data are provided as a Source Data file.
