Fig. 4: Mice lacking GLTP in oligodendrocytes exhibit ER pathology in myelin.
From: Nonvesicular lipid transfer drives myelin growth in the central nervous system
a Membrane rings in the inner tongue at the spinal cord of P14 Gltp cKO (Cnp-Cre, Gtlpflox/flox). Arrows: thin rings; arrowheads: thick rings; Star: a comet tail-like structure associated with the ring; double arrowheads: rings in the outer tongue b Ratio (mean ± SD) of inner tongue containing rings: 0.08\(\pm\)0.13% of inner tongues in the optic nerve of Cnp-Cre (Cre on), 19.34\(\pm\)3.65% in the optic nerve of Cnp-Cre, Gtlpflox/flox (cKO on), 33.90\(\pm\)6.87% in the spinal cord of Cnp-Cre, Gtlpflox/flox (cKO sc) (n = 3 mice for each condition, from 436 + 537 + 543 inner tongues of “Cre on”, 508 + 525 + 531 of “cKO on”, 589 + 585 + 664 of “cKO sc”, One-way ANOVA: F (2, 6) = 42.77, Tukey’s post-hoc test: Cre on vs. cKO on **p = 0.0046, cKO on vs. cKO sc *p = 0.0173) c Volume EM of the optic nerve from Gltp cKO. Ring membrane is segmented in green, and 3D reconstruction of ring from a 1.5 µm-thick stack (31 slices with 50 nm interval), from dark green to light green across the stack. Scale cube: 1 × 1 × 1 µm. d Amount of ER decrease in the presence of rings, suggested by the ratio (mean ± SD) of inner tongue containing the ER: 90.34\(\pm\)3.33% of ring- vs. 44.69\(\pm\)6.01% of ring+ inner tongues in optic nerve of Cnp-Cre, Gtlpflox/flox (cKO on), 90.67\(\pm\)5.47% of ring- vs. 51.31\(\pm\)2.78% of ring+ inner tongues in the spinal cord of Cnp-Cre, Gtlpflox/flox (cKO sc) (n = 3 mice for each condition, one-way ANOVA: F (3, 8) = 86.07, Sidak’s multiple comparisons test: ****p < 0.0001) Scale bars: 1 µm (a), 150 nm (a zoom-in view) 200 nm (c). Source data are provided as a Source Data file.
