Fig. 5: FCS analysis of C9-CF binding to reconstituted mononucleosomes.
From: Epigenetic modulation via the C-terminal tail of H2A.Z
A Representative normalized autocorrelation functions (ACF-s) of C9-CF are shown; the correlation curves were acquired in the absence (red) or presence (green) of nucleosomes, at 700 mM NaCl concentration. Fits assuming triplet transition and a single diffusing species for a sample without nucleosomes and two diffusing populations with nucleosomes were used (black solid lines). The gray line indicates the fit excluding triplet state transition. τD1 and τD2 are the diffusion times of the fast and slow components, characterizing the diffusion of freely diffusing and nucleosome-bound C9-CF peptides, respectively. The ACF-s shifted toward slower diffusion times indicating the presence of a nucleosome-bound fraction. B Fit residuals. C Slow fraction of C9-CF. The peptide was incubated with nucleosomes at 5 mM or 700 mM NaCl concentration, with Cy5-labeled Widom-sequence at 5 mM or 700 mM NaCl, or with genomic DNA at 5 mM NaCl. The slow fraction ρ2 was calculated in ACF fits as described in the “Methods” and presented as a box-and-whisker plot.
