Fig. 1: FNDC4 expression is elevated during cardiac I/R injury in a HIF1α-dependent manner. | Nature Communications

Fig. 1: FNDC4 expression is elevated during cardiac I/R injury in a HIF1α-dependent manner.

From: FNDC4 alleviates cardiac ischemia/reperfusion injury through facilitating HIF1α-dependent cardiomyocyte survival and angiogenesis in male mice

Fig. 1

a The hearts with or without I/R injury were harvested for western blot (n = 6). b The left ventricles from ischemic heart disease (IHD) patients or donors were prepared for western blot (n = 6). c, d Neonatal rat cardiomyocytes (NRCMs) and human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) with or without sI/R injury were prepared for western blot (n = 6 independent experiments). e, f Correlations between cardiac Fndc4 mRNA and serum cardiac isoform of tropnin T (cTnT) or fractional shortening (FS) in I/R-stressed mice (n = 20). g Plasma FNDC4 levels in sham- or I/R-stressed mice (n = 20). h, i Correlations between plasma FNDC4 and serum cTnT or FS in I/R-stressed mice (n = 20). j Plasma FNDC4 levels in acute myocardial infarction (AMI) patients or healthy controls (n = 26 for donors and n = 42 for AMI patients). k Correlation between plasma FNDC4 and high-sensitivity tropnin I (hs-TnI) in AMI patients (n = 42). l Plasma FNDC4 levels in AMI patients at admission and after PCI surgery (n = 42). m FS in AMI patients with low or high FNDC4 levels at 1, 6 and 12 months post-PCI surgery (n = 10 for low FNDC4 group and n = 16 for high FNDC4 group). n The clinical outcome of AMI patients with low or high FNDC4 levels after PCI surgery (n = 21). o The hearts with or without HIF1α inhibition were prepared for western blot (n = 6). p NRCMs with or without HIF1α inhibition were prepared for western blot (n = 6 independent experiments). Data were presented as the mean ± S.D., and analyzed using an unpaired two-tailed Student′s t-test. For the analysis in (e–k), Pearson’s correlation analysis was used. For the analysis in (l), a paired two-tailed Student′s t-test was performed. For the analysis in (m), repeated measures ANOVA was conducted. For the analysis in (n), a two-tailed χ2 test with Yates’ correction was used, χ2 = 2.713, df = 1, z = 1.647. For the analysis in (o, p), one-way ANOVA followed by Tukey post hoc test was used. *P < 0.0001. Source data are provided as a Source Data file.

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