Fig. 3: The influence of surface-bound DNA on transfection efficiency (TE) and cell viability. | Nature Communications

Fig. 3: The influence of surface-bound DNA on transfection efficiency (TE) and cell viability.

From: Structuring lipid nanoparticles, DNA, and protein corona into stealth bionanoarchitectures for in vivo gene delivery

Fig. 3

a Schematic representation of LNPs loaded with functional DNA (f-DNA) in green, and non-functional DNA (nf-DNA) in yellow. These two versions of LNPs were respectively decorated with f-DNA and nf-DNA, resulting in the formation of LNPi and LNPi nf-DNA. b TE was assessed in HEK-293 cells through the luciferase assay, quantified as relative light units (RLU) per milligram of proteins (n = 6 independent measurements), and (c) through the green fluorescence protein assay (n = 2 independent measurements), measured as mean fluorescence intensity (MFI). d Cell viability data are presented as a percentage of viable cells (n = 6 independent measurements). Statistical analysis was performed by one-way ANOVA test, followed by Tukey’s multiple-comparison test. P-values < 0.05 are reported. Color code: LNP (gray); LNPi (green); LNPi nf-DNA (yellow). Data are presented as mean values ± standard deviation. Source data are provided as a Source Data file.

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