Fig. 6: Investigation of LNP-cell interactions.
a TE as a function of HP concentration (% vol), (b) TE of selected conditions (i.e. both particles at 0%HP and the one exhibiting highest TE in presence of HP, n = 6 independent measurements), (c) cell viability as function of HP concentration (% vol), and (d) cell viability of selected conditions (i.e. both particles at 0%HP and the one exhibiting highest TE in presence of HP, n = 3 independent measurements). In Panels a, c, statistical significance is evaluated by two-sided Student’s t-test at each HP concentration. Statistical analysis in Panels b, d was performed by one-way ANOVA, followed by Tukey’s multiple-comparison test. P-values < 0.05 are reported. Confocal images of (e) LNP, (f) LNPi and, (g) coronated LNPi after administration to HEK-293 cells at 37 °C. Green and red channels indicate lipids, and lysosomes respectively, nuclei are stained in blue. Magnifications are displayed in (h–j). k Quantification of colocalization in terms of the Pearson’s coefficient. l number of particles per cell. m iMSD vs. time lag curves. n Offset parameter, σ02, (o) anomalous diffusion coefficient (α), (p) short-range diffusion constant (Dm), (q) long-range diffusion constant (DM). Parameters reported in panels n-q are determined through iMSD plot fitting. The datasets were made of n = 10 images per class. Statistical analysis was performed by one-way ANOVA test, followed by Tukey’s multiple-comparison test. Data are presented as mean values ± standard deviation. P-values < 0.05 are reported. Source data are provided as a Source Data file.
