Fig. 9: Biodistribution of LNP, LNPi and coronated LNPi in vivo.

Nanoparticle accumulation in the liver, lungs, spleen, kidneys, and heart was estimated by fluorescence associated with fluorescently labeled nanoparticles (TexasRed). Images of organs from a representative mouse per group (n = 4/group of 8-10 weeks-old C57BL/6 female mice), 3 h after injection with (a) LNP, (b) LNPi, and (c) coronated LNPi. d Quantification of the fluorescence associated with the observed organs, reported as total flux (photons/sec). The signal from non-treated mice (N.T.) is reported as a control. For each organ, statistical analysis was performed by one-way ANOVA, followed by Tukey’s multiple-comparison test. P-values < 0.05 are reported. Data are presented as mean values ± standard deviation. Source data are provided as a Source Data file.