Fig. 5: r&bLED together with F-gel synergistically accelerates angiogenesis via improving MRC.
From: A wearable and stretchable dual-wavelength LED device for home care of chronic infected wounds
a Schematic diagram of the mechanisms of rLED combined with F-gel to improve mitochondrial function. b Intracellular NAD(H) levels, CIV activity and ATP level in HUVECs after cocultured with or without LPS, F-gel (NADH concentration,100 μM) and rLED with different energy densities (rLED-L, 1 J/cm2; rLED-M, 3 J/cm2; rLED-H, 5 J/cm2). c, f Representative EdU staining micrographs (c) and statistical analysis (c) of positive ratios of HUVECs with different treatments for 24 hours. d, g, Wound scratch assay of HUVECs observed by an optical microscope (d) and the calculation of relative wound area (g). e, h Tube formation of HUVECs (e) and the Statistical analysis of capillary length (h) after treatment. i, k, m Intracellular ROS (i), mitochondrial membrane potential (ΔΨM) (k) and NF-κB p65 nuclear translocation (m) of HUVECs observed by CLSM after cocultured with or without LPS and different treatments. j, l, n Statistical analysis of intracellular ROS (j), mitochondrial membrane potential (ΔΨM) (l) and NF-κB p65 nuclear translocation (n) of HUVECs. o, q, HUVECs apoptosis triggered by LPS (100 ng/mL, 48 h) and TNF-α (80 ng/mL, 48 h) analyzed by Annexin V/PI double staining. p, r, Quantitative analysis of LPS (p) and TNF-α (r) triggered apoptosis in HUVECs. Values in b, f–h, j, l, n represent the mean ± s.d. (n = 5 biologically independent samples in each group). Data are presented as mean values ± SEM. Statistical significance and P values were determined by one-way ANOVA followed by Tukey’s multiple comparison test. Panel a was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
