Fig. 1: Strategy for identifying dimethyl-labeled peptides.
From: Using in vivo intact structure for system-wide quantitative analysis of changes in proteins
Three mice per each age group (ranging 6 months to 15 months) were used for AD (APPNL-F) and NC (C57BL6/J). The first step of the CPP workflow consists of three sub-steps that were conducted via perfusion: (i) blood was washed by PBS, (ii) tissue was fixed by formaldehyde, and (iii) exposed lysine sites of the native proteins were labeled with light-dimethylation ([CD2H]2). Proteins from each of the seven organs were extracted and digested separately with chymotrypsin, after which the newly exposed lysine sites were labeled with heavy dimethylation ([C13D3]2). Created in BioRender. Kim, H. (2022) BioRender.com/d78w669.
