Fig. 3: Variability of the conformational changes depending on the tissues.

A Two representative peptides (ILETQKQF and GIQKELQF) were shown representatively. The normalized values were utilized to fit spline models. The accessibility of each peptide for both NC (blue) and AD (red) exhibited significantly distinct patterns from 6 to 15 months (Benjamin Hochberg, adjusted P-values = 0.0036 (brain), 0.0282 (muscle) for ILETQKQF, adjusted P-values = 0.0029 (brain), 0.0028 (muscle) for GIQKELQF). B Venn diagram shows the number of peptides exhibiting significant differences in the trend of accessibility changes between NC and AD. There were no peptides from AD that showed a significant difference in accessibility changes compared to NC in all 7 tissues during the period from 6 to 15 months. The value of zero was not indicated. C–H During AD progression, 10 common peptides exhibited distinct patterns in accessibility between NC and AD in different four tissues. The variabilities for the structural changes in each tissue were calculated based on the value of brain using the formula: (fold-change of other tissue - fold-change of brain) / fold-change of brain at 6 mo (C), 9 mo (D), 12 mo (E), and 15 mo (F). Only the first three amino acids were shown. AGTAEAIKAL of Gatd3 (G) and GIQKELQF of Ldha (H) showed a difference in the magnitude of accessibility change in muscle and spleen compared to that in the brain as AD progressed. The number indicates the position of the labeled lysine site within the sequence. I. Enriched KEGG pathways with 10 proteins. P-value were corrected with Bonferroni. Source data are provided as a Source Data file.