Fig. 7: The physical interactions of the proteins in M3 of brain.

A Forty-five proteins in the brain dataset were physically interacted. Each node indicates a protein. The ring color of the node indicates the terms that the protein is associated with. The size of node represents the number of significantly changed lysine sites, with very small nodes indicating no significantly changed peptides, small nodes indicating one significantly changed peptide, medium nodes indicating 2–4 significantly changed peptides, and large nodes indicating more than 4 significantly changed peptides. B The structure of the Plp1-Mag complex was predicted using AlphaFold-Multimer. The structure in dark pink is Plp1 and the structure in light purple is Mag. FSKNYQDY of Plp1 and YFNSPYPKNYPPVVF of Mag were presented in green and red, respectively. The right panel is an enlarged view of the complex on the left. The distance between alpha-carbons of two lysine sites was 13.9 Å. C, D Structural changes in adjacent peptide regions with potential binding, with variation of the accessibility of site in AD (pink) and NC (green) for FSKNYQDY (C) and YFNSPYPKNYPPVVF (D). Three mice per age were used for both NC and AD. Error bars indicate the mean ± standard deviation. Source data are provided as a Source Data file.