Fig. 4: Cellular uptake and cytotoxic effects of Ru(II)-OH, NP2, and NP4 against 143B and K7M2 cells under 21% O2 and 1% O2 conditions.
a Time-dependent confocal laser scanning microscopy (CLSM) images of the cellular uptake of NP4-Cy5.5 in 143B cells or 143B multicellular tumor spheroids. The cell nucleus was stained with 4’, 6-diamidino-2-phenylindol (DAPI), and the cell cytoskeleton was stained with Alexa-488. b Time-dependent cellular uptake of NP4-Cy5.5 in 143B cells determined by flow cytometry. c Quantification of the cellular uptake of NP4-Cy5.5 from (b) (n = 3 independent experiments). d Representative drug response curves of Ru(II)-OH, NP2, and NP4 against 143B and K7M2 cells in a 1% or 21% O2 atmosphere, (n = 3 independent experiments). e Representative CLSM images of 143B cells and 143B multicellular tumor spheroids under 21% O2 or 1% O2 conditions treated with of Ru(II)-OH, NP2, and NP4 (10 μM Ru) for 24 h and stained with cell live (calcein AM, green) and cell death (propidium iodide, red). f Flow cytometry plots of 143B cells under 1% O2 conditions treated with of Ru(II)-OH, NP2, and NP4 (10 μM Ru) for 12 h and stained with Annexin V-FITC and propidium iodide. g Determination of the apoptosis rate from (f) (n = 3 independent experiments). Data are presented as mean ± standard deviation (SD). Statistical significance between every two groups was calculated by T-test, the statistical test used was two-sided.
