Fig. 5: OSH15, a transcriptional inhibitor, is required for OsSTP28-regulated gibberellin catabolism.
From: Sugar transporter modulates nitrogen-determined tillering and yield formation in rice
a Volcano plot of DEGs (the marker genes of tiller development) based on RNA-seq data from WT and stp28 mutant (stp28-3 was used for RNA-Seq in this study) under LN or HN supply. down, down-regulated genes; up, up-regulated genes; No Diff, no significant difference. b Expression abundances of OSH15 in WT, stp28 lines under LN or HN supply. Data are mean ± SD (n = 5 biological replicates). c OSH15 directly binds to motif regions of OsGA2ox3/5/8/9 promoter in EMSA. OSH15 binding site was indicated with red triangle in the respectively promoter model. The results are representative of three independent experiments. d Transactivation assays. OSH15 displays transcriptional repression activity to GA2ox3/5/8/9 promoter-LUC in rice protoplasts. Data are mean ± SD (n = 9 biological replicates. Significant difference was determined by two-sided Student’s t-test. e Expression abundance of OsGA2ox3/5/8/9 in WT and osh15 lines under LN or HN supply. Data are mean ± SD (n = 4 biological replicates). f–h, GA2ox activities (f), phenotype (g) and tiller number (h) and of WT and osh15 lines under LN or HN supply. In f, h, boxes show the first quartile, median, and third quartile; whiskers show the minimum and maximum values. n = 4 biological replicates in (f), and n = 9 individual plants in (h). Scale bar, 20 cm. In (b, e, f, h), Different letters indicated significant differences (P < 0.05, one-way ANOVA, Tukey’s HSD test).
