Fig. 5: Signaling pathway screening reveals downstream candidates responsible for ISC aging phenotype.

a, b Quantification of the number of esg⁺NRE⁻Pros⁻ ISCs (a) and NRE⁺ EBs (b) per ROI in 10d flies with indicated genotypes. c The ratio of Pre-EE to total esg⁺ cells per ROI in 10d flies with indicated genotypes. d The percentage of differentiated cells and esg⁺ cells to total cells in 10d flies with indicated genotypes. e, f Quantification of the number of esg⁺NRE⁻Pros⁻ ISCs (e) and NRE⁺ EBs (f) per ROI in 40d flies with indicated genotypes. The dark dotted line represents the mean value from 10d control flies. g The ratio of Pre-EE to total esg⁺ cells per ROI in 40d flies with indicated genotypes. The dark dotted line represents the mean value from 10d control flies. h The percentage of differentiated cells and esg⁺ cells to total cells in 40d flies with indicated genotypes. The purple line represents the mean percentage value of differentiated cells from 10d control flies. The green line represents the mean percentage value of esg⁺ cells from 10d control flies. i The percentage of ECs and EEs to total differentiated cells in 40d flies with indicated genotypes. The red line represents the mean percentage value of ECs from 10d control flies. The blue line represents the mean percentage value of EEs from 10d control flies. ROI size 84,100 μm². For dot plots, bars are mean ± SD. For box plots, box shows median, 25th and 75th percentiles and whiskers represent minima and maxima. Each group was compared to the control group in all analyses. Statistics were measured by one-way ANOVA. Each dot represents one ROI in (a–c) and (e–g). From left to right, n = 18, 9, 12, 15, 17, 15, 12, 17, 16, 23, 30, 16 and 25, respectively in (a–d), n = 18, 13, 32, 21, 38, 42, 19, 29 and 24, respectively, in (e–g) and n = 18, 13, 32, 21, 27, 33, 19, 29 and 24, respectively in (h-i). Source data are provided as a Source Data file.