Fig. 6: RAB7 regulates ISC differentiating direction downstream of InR.

a–d Representative immunofluorescence images of midguts with ISC-GFP (green), NRE-lacZ (red) and Pros (white) staining from 10d flies expressing UAS-GFP (used as control, a), InR^CA (b), Rab7^CA (c), and InR^CA, Rab7^CA (d) in driven by ISC^ts-Gal4. e–g Quantification of the number of esg⁺NRE⁻Pros⁻ ISCs (e), NRE⁺ EBs (f) and esg⁺Pros⁺ pre-EEs (g) per ROI of midguts in experiments (a–d). h The ratio of pre-EE cells to esg⁺ cells per ROI with indicated genotypes of midguts in experiments (a–d). i The percentage of differentiated cells and esg⁺ cells to total cells per ROI in indicated genotypes of midguts in experiments (a–d). j The percentage of ECs and EEs to total differentiated cells in indicated genotypes of midguts in experiments (a–d). ROI size 84,100 μm². DAPI-stained nuclei (blue). Yellow arrows indicate esg⁺Pros⁺ pre-EE cells. Scale bar, 25 μm. For dot plots, bars are mean ± SD. For box plots, box shows median, 25th and 75th percentiles and whiskers represent minima and maxima. Statistics were measured by one-way ANOVA. Each dot represents one ROI in (e–h). From left to right, n = 18, 17, 15, and 15, respectively in (e-j). Source data are provided as a Source Data file.