Fig. 8: RAB7 is down-regulated in aged human ISCs. | Nature Communications

Fig. 8: RAB7 is down-regulated in aged human ISCs.

From: Nutrient-sensing alteration leads to age-associated distortion of intestinal stem cell differentiating direction

Fig. 8

a, b Immunofluorescence images of human duodenum samples section with RAB7 (green) and SOX9 (red) staining from young (a) and old (b) groups. c Quantification of the number of RAB7+ endosomes in SOX9-labeled ISCs of human samples from the young group (n = 45) and the old group (n = 45). Each dot represents one SOX9+ ISC. d, e Schematic of the proposed model. InR is aberrantly upregulated in ISCs upon aging, which leads to reduced differentiation into ECs through the down-regulating RAB7-SOX21A axis. Due to the SC loop, ISCs exhibit a change of differentiating direction. DAPI-stained nuclei (blue). Yellow frames indicate SOX9+ ISCs. Scale bar, 5 μm. Bars are mean ± SD. Statistics were measured by two-tailed, unpaired student’s t-test. Source data are provided as a Source Data file.

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