Fig. 4: Supernumerary subunits in P. denitrificans complex I.
From: Structure of the turnover-ready state of an ancestral respiratory complex I
a The locations of subunits NDUFS4 (green), CI-PIMT (purple), NDUFA12 (blue) and NDUFS6 (red). b Secondary structure comparison of subunits NDUFS4, NDUFS6 and NDUFA12 with bovine complex I PDB-7QSL20, shown in their arrangement in the structure. c Comparison of electrostatic surface of free-PIMT and CI-PIMT showing binding of CI-PIMT is governed by electrostatic interactions with NDUFS3. d Comparison of key residues involved in cofactor and substrate binding in the functional PIMT from Pyrococcus furiosus PDB-1JG467 and CI-PIMT. e Cross-section of the Pd-CI hydrophilic domain showing that substrate binding in PIMT is obstructed in the complex.
