Fig. 2: Optical characterization.
From: Fast and light-efficient remote focusing for volumetric voltage imaging
a Averaged measured point spread functions from 0.1 μm diameter fluorescent beads over an 80 μm defocus range at the center and the edge of the FOV. b Normalized average maximal intensity from PSFs at each z-position. Intensity stays >80% over a range of ~100 μm in the center of the FOV and 60 μm at the edge. Shaded area designates standard error of the mean (s.e.m.). The PSF at each z-position in a and b was calculated by averaging over n = 26–127 beads. c Maximum drive frequency limited by the voice coil motor as a function of defocus.
