Fig. 1: Construction of fungal cell and the schematic of the affinitymolecular assay for fungal detection.
From: Affinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a
a Structure of fungal cell walls. b Chemical structure of chitin. c Electrostatic potential surface view of chitin with CBP. d Coupling of carboxyl magnetic beads and CBP. e Flow diagram of fungal detection by affinity molecular assay: (1) Fungal capture using MB-protein complexes: the complexes were added to samples and incubated for 30 min to capture fungal cells. (2) Lysis of captured fungi with lysis buffer at 55 °C for 5 min, followed by incubation with silicone hydroxyl magnetic beads at room temperature (RT) for 10 min to extract genomic DNA. (3) Nucleic acid detection using a one-pot detection system or qPCR assay. The sample-to-result turnaround time was within 2.5 h, including 30–50 min (related to the number of samples) of hands-on time, and 40 min of RPA-CRISPR/Cas12a reaction, 50 min beads-capture, and incubation. Source data are provided as a Source Data file.
