Fig. 4: Establishment of the One-Pot detection system for C. albicans.
From: Affinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a
a The target sequences of C. albicans CHS1 gene and the locations of RPA primers and crRNA in this assay. b The scheme of the RPA-CRISPR/Cas12a one-pot visual detection system. c, e Verification of the sensitivity of the one-pot detection system with 10-fold serially diluted C. albicans genomic DNA. d, f The specificity of the one-pot detection system with C. albicans genomic DNA. c, d The real-time fluorescence intensity alteration for different concentrations of targets. e, f The directly Visualized fluorescence signals detection under blue light. g, h Sensitivity and specificity detection with C. albicans genomic DNA using qPCR. The reaction volume of one-pot reactions in (c–f) was 30 μL, and for qPCR in (g, h) was 20 μL. The corresponding sample volumes added to both reactions were identical. NC: represents reactions without substrate. For c–h, each experiment was repeated three times, with one representative result shown. Source data are provided as a Source Data file.
