Fig. 6: Feasibility of the affinity molecular method in a murine model of disseminated C. albicans infection.

Mice were randomly divided into three groups: a blank control group (blank), animals infected and treated with vehicle (PBS alone control group), and mice infected and treated with fluconazole (treatment group). a Schematic depiction of the experiments in BALB/c mice. b Kidney fungal burdens in the murine model of invasive C. albicans infection treated with PBS and fluconazole (Blue indicates the PBS control group, and red indicates the fluconazole treatment group), respectively. c, d Detection of whole blood from mice using the affinity molecular method: c The fluorescence intensity of the one-pot detection system readout by a fluorescence monitor (Blue indicated the PBS control group, red indicated the fluconazole treatment group, and green was the negative control results). d Visual detection of fluorescence signals under blue light. The data are presented as mean in b and mean ± s.d. in (c), n = 4 in each group, n indicates the number of individual mice. For b, c, statistical significance was analyzed using a two-tailed Student’s t-test. NC: represents reactions without substrate. e Histological analyses of kidney sections: Left, HE staining; Right, PAS staining. Arrowheads in the PAS staining image indicated the C. albicans spore got dyed dark red. n.s.: no significant difference. Scale bars: 20 µm. Source data are provided as a Source Data file.