Fig. 3: ASB2, ITGAV, and ITGB3 are Th2-specific genes.
From: Fine-tuning levels of filamins a and b as a specific mechanism sustaining Th2 lymphocyte functions
a Mass spectrometry semi-quantitative analyses of integrin abundance in cell extracts of ctrl and ASB2 cKO Th2 lymphocytes (n = 9 replicates). Full data are presented in Supplementary Data 1. b Heatmap showing the expression of genes encoding the α and β integrin subunits (ITGA and ITGB, respectively) in naive, Th2, Th1, and Th17 cells (analyzed from ref. 30). RPKM intensities were log10 transformed and are displayed as colors ranging from blue to red as shown in the key. c RNA polymerase II (Pol II), H3K27ac, H3K4me1 and H3K27me3 signals across the ASB2, ITGAV and ITGB3 loci. The identified cis-regulatory regions are highlighted (GSE14458630); d Representative flow cytometry and quantification of cell surface expression of αV and β3 integrin subunits in naive CD4+ T cells and in Th1 (CD4+CXCR3+CCR6−CRTH2−FoxP3−), Th2 (CD4+CXCR3−CCR6−CRTH2+FoxP3−), Th17 (CD4+CXCR3−CCR6+CRTH2−FoxP3−) and Treg (CD4+CXCR3−CCR6−CRTH2−FoxP3+) lymphocytes of human PBMCs. e Quantification of cell surface expression of αV and β3 integrin subunits in naive and in vitro generated human Th2 lymphocytes. Data are mean ± SEM of biological replicates. Sample size: ctrl = 9 and cKO = 9 for (a); n = 6 for (d); and n = 4 for (e). p values were calculated using the two-sided Mann–Whitney t-test except in (d) where the Wilcoxon matched-pairs signed rank test was used. Source data are provided as a Source Data file.
