Fig. 4: ASB2α-mediated degradation of FLNa and FLNb confers specific migration properties to Th2 lymphocytes.
From: Fine-tuning levels of filamins a and b as a specific mechanism sustaining Th2 lymphocyte functions
Ctrl and ASB2 cKO Th2 lymphocytes seeded onto vitronectin-coated slides were imaged by time-lapse microscopy. Representative images (left) and individual tracks (right) of one experiment out of 5 (a), percentages of migrating cells (b), track length (c), mean scanned area (d), persistence coefficient (e), average velocities of all the cells (left panel) or cells with a track length > 50 µm (right panel) (f), elongation coefficient and average sphericity (g) are shown. Scatter plots showing correlation data for elongation coefficient and track length (h) or average velocity (i) of ctrl or ASB2 cKO Th2 lymphocytes. Linear regression-fit curves are shown as red lines. Scale bar, 20 µm. In (a–g), Data are mean ± SEM of 5 biological replicates. Sample size: ctrl = 1070 and cKO = 916 for (c); ctrl = 1070 and cKO = 916 for (d); ctrl = 1070 and cKO = 916 for (e); ctrl = 1070 and cKO = 916 for (f) (left); ctrl = 685 and cKO = 470 for (f) (right); ctrl = 92,674 and cKO = 85,399 for (g); ctrl = 1070 and cKO = 916 for (h); and ctrl = 1070 and cKO = 916 for (i). p values were calculated using the two-sided Mann–Whitney t-test. In (h, i), correlations between nonparametric variables were evaluated using Spearman rank correlation test (r). Source data are provided as a Source Data file.
