Fig. 5: Migration properties of ctrl and ASB2 cKO Th2 lymphocytes following MnCl2-induced integrin activation or αVβ3 inhibition.
From: Fine-tuning levels of filamins a and b as a specific mechanism sustaining Th2 lymphocyte functions
Ctrl and ASB2 cKO Th2 lymphocytes were either untreated or treated with MnCl2 (a–d) and treated with anti-αV and anti-β3 integrin blocking antibodies (Ab) or their corresponding isotypic controls (iso) (e–h), and allowed to migrate onto vitronectin-coated slides. Cells were imaged by time-lapse microscopy. Individual tracks (a, e), percentages of migrating cells (b, f), track length (c, g), average velocities of all the cells (d, h) are shown. Data are mean ± SEM of 4 and 5 biological replicates for ctrl and cKO Th2 lymphocytes respectively. Sample size: ctrl = 1809, ctrl+MnCl2 = 2344, cKO = 1611, and cKO + MnCl2 = 1717 for (c, d); and ctrl + iso = 2768, ctrl +Ab = 2803, cKO + iso = 2261, and cKO + Ab = 1989 for (g, h). p values were calculated using the two-sided Mann–Whitney t-test. Source data are provided as a Source Data file.
