Fig. 6: Deletion of ASB2 in hematopoietic cells attenuates OVA-airway inflammation in mice.
From: Fine-tuning levels of filamins a and b as a specific mechanism sustaining Th2 lymphocyte functions
Ctrl and ASB2 cKO mice were submitted to OVA-airway inflammation (OVA) as indicated in the online methods. Ctrl mice treated with PBS were used as controls. a Inflammation of the lungs assessed using hematoxylin and eosin (HE) staining of lung sections to analyze the infiltration of inflammatory cells (0–12-point scale), Masson’s trichrome (MT) staining of lung sections to visualize and quantify collagen deposits (µm2/µm) (collagen area/bronchus perimeter), and periodic acid Schiff (PAS) staining to visualize and quantify mucus production. b Data represents the numbers of CD45+ cells in the lungs. c Numbers of CD45+Siglec-F+CD11c− (eosinophils, left panel) and CD45+Siglec-F+CD11c+ (alveolar macrophages; right panel). Representative flow cytometry plots for CD11c and Siglec-F within a CD45+ gated (middle panel) in the lungs of OVA-treated mice. d Relative expression of eotaxin 2 mRNA in the lung lysates assessed by RT-qPCR. e Numbers of CD45+CD4+ in the lungs. f Percentage of ST2+ cells in CD4+ cells, representative flow cytometry plots for ST2 and CD4 within a CD4+ gate, and numbers of CD45+CD4+ST2+ in the lungs. g Data represents the percentage of CD4+ in CD45+ cells and the percentage of ST2+ in CD4+ cells in the BAL fluids. h Relative expression of IL-4, IL-5 and IL-13 mRNA in the lung lysates. i Data represents the percentage of IL-4+, IL-5+ or IL-13+ in CD4+ cells and in ST2+CD4+ cells in the lungs. j Production of IL-4, IL-5, and IL-13 measured by ELISA after antigen restimulation (OVA) or not (−) of cells of the lung draining lymph nodes of mice submitted to OVA-induced airway inflammation. k Expression of FLNa was analyzed by intracellular flow cytometry in CD45+CD4+ST2+ cells from the lungs of ctrl or ASB2 cKO mice submitted to OVA-induced airway inflammation. l Intensities of FLNa calculated using MaxQuant quantitative metrics in cell extracts of CD45+CD4+ST2+ living cells sorted from the lungs of control or ASB2 cKO mice submitted to OVA-induced airway inflammation. Data are mean ± SEM of biological replicates. Sample size: ctrl+PBS = 6, ctrl + OVA = 14, and cKO + OVA = 17 for (a) (histological score); ctrl+PBS = 3, ctrl + OVA = 14, and cKO + OVA = 17 for (a) (PAS+ bronchi); ctrl + PBS = 3, ctrl + OVA = 10, and cKO + OVA = 15 for (a) (collagen deposition-left); ctrl + PBS = 30, ctrl + OVA = 249, and cKO + OVA = 275 for (a) (collagen deposition-right); ctrl + PBS = 6, ctrl + OVA = 34, and cKO + OVA = 33 for (b); ctrl + PBS = 5, ctrl + OVA = 26, and cKO + OVA = 28 for (c); ctrl + OVA = 19 and cKO + OVA = 22 for (d); ctrl + PBS = 6, ctrl + OVA = 32, and cKO + OVA = 31 for (e); ctrl + PBS = 5, ctrl + OVA = 25, and cKO + OVA = 24 for (f); ctrl + OVA = 21 and cKO + OVA = 27 for (g) (left); ctrl + OVA = 10 and cKO + OVA = 12 for (g) (right); ctrl + OVA = 20 and cKO + OVA = 24 for (h); ctrl + OVA = 14 and cKO + OVA = 14 for (i) (left); ctrl + OVA = 14 and cKO + OVA = 14 for (i) (middle); ctrl + OVA = 9 and cKO + OVA = 8 for (i) (right); ctrl + OVA = 7 and cKO + OVA = 8 for (j); ctrl + OVA = 20 and cKO + OVA = 19 for (k); and ctrl + OVA = 3 and cKO + OVA = 3 for (l). p values were calculated using the two-sided Mann–Whitney t-test. Scale bar, 200 µm. Source data are provided as a Source Data file.
