Fig. 7: Deletion of ASB2 in hematopoietic cells attenuates HDM-asthma in mice and intrinsic loss of ASB2 in Th2 cells is sufficient to impede airway inflammation.

a–g Ctrl and ASB2 cKO mice were submitted to HDM-allergic airway inflammation (HDM) as indicated. a HE, MT, and PAS staining of lung sections to analyze the infiltration of inflammatory cells and inflammatory scores. b Data represents the numbers of CD45⁺ cells in the lungs. c Data represents the numbers and the percentages of Siglec-F⁺CD11c⁻ in CD45⁺ cells in the lungs. d Data represents the percentages of Siglec-F⁺CD11c⁻ in CD45⁺ cells in the BAL fluids. e Data represents the numbers of CD4⁺CD45⁺ cells, the percentages of ST2⁺ in CD4⁺ cells, and the numbers of ST2⁺CD4⁺CD45⁺ cells in the lungs. f Data represents the percentages of CD4⁺ in CD45⁺ cells and the percentages of ST2⁺ in CD4⁺ cells in the BAL fluids. g Expression of FLNa was analyzed by intracellular flow cytometry in CD45⁺CD4⁺ST2⁺ cells from the lungs of ctrl or ASB2 cKO mice submitted to HDM-induced asthma. h–q OVA-specific Th2 lymphocytes generated from control or ASB2 cKO OT2 mice were transferred to C57Bl/6 recipients that were subsequently submitted to daily OVA inhalations to induce airway inflammation. Analysis was performed 24 h after the 5th (H, O) or after the 1st OVA inhalation (p, q). h HE staining of lung sections. i MT staining of lung sections. j PAS staining of lung sections. k Data represents the numbers of CD45⁺ in the lungs. l Data represents the numbers and the percentages of Siglec-F⁺CD11c⁻ in CD45⁺ cells in the lungs. m Data represents the percentages of Siglec-F⁺CD11c⁻ in CD45⁺ cells in the BAL fluids. n Data represents the numbers of Vβ5⁺Vα2⁺CD4⁺CD45⁺ cells in the lungs. o Data represents the percentages of Vβ5⁺Vα2⁺ in CD4⁺ cells in the lungs and the BAL fluids. p Data represents the numbers of Vβ5⁺Vα2⁺CD4⁺CD45⁺ cells in the lungs. q Data represents the percentages of Vβ5⁺Vα2⁺ in CD4⁺ cells in the lungs and the BAL fluids. Data are mean ± SEM of biological replicates. Sample size: ctrl = 12 and cKO = 12 for (a–c); ctrl = 12 and cKO = 10 for (d); ctrl = 12 and cKO = 12 for (e); ctrl = 12 and cKO = 10 for (f); ctrl = 12 and cKO = 12 for (g); ctrl = 10 and cKO = 6 for (k); ctrl = 9 and cKO = 6 for (l); ctrl = 10 and cKO = 6 for (m, n); ctrl = 10 and cKO = 6 for (o) (left); ctrl = 8 and cKO = 4 for (o) (right); ctrl = 16 and cKO = 12 for (p); ctrl = 16 and cKO = 12 for (q) (left); and ctrl = 5 and cKO = 4 for (q) (right). p values were calculated using the two-sided Mann–Whitney t-test. Scale bar, 200 µm. Source data are provided as a Source Data file.