Fig. 2: The synthesis of PS-CHO and ATRA-SS-ATRA, ALDH-responsiveness of PS-CHO and Preparation of PS-CHO@ATRA-SS-ATRA. | Nature Communications

Fig. 2: The synthesis of PS-CHO and ATRA-SS-ATRA, ALDH-responsiveness of PS-CHO and Preparation of PS-CHO@ATRA-SS-ATRA.

From: Self-assembled aldehyde dehydrogenase-activatable nano-prodrug for cancer stem cell-enriched tumor detection and treatment

Fig. 2

a The synthesis route of PS-CHO and ATRA-SS-ATRA. The condition and agents: a. NaBH4, I2, 60 °C; b Hexamethylenetetramine, CF3COOH, 80 °C; c Et3N, 2-(3-cyano-4,5,5-trimethylfuran-2(5H)- ylidene) malononitrile; d Dess-Martin Reagent. r.t.; e DCC, DMAP, 0-r.t, overnight. b The HPLC profiles for PS-CHO, PS-COOH and PS-CHO treated with ALDH (50 μg/mL) in PBS for 4 h. c Fluorescence spectra of PS-CHO, PS-COOH and PS-CHO incubated with ALDH (50 μg/mL) for 4 h. d Fluorescence variation PS-CHO incubated with ALDH (50 μg/mL) at different times (0–4 h). e Total ROS generation for PBS, PS-CHO, PS-COOH and PS-CHO with ALDH (50 μg/mL) by DCFH assay (40 mW/cm2, 4 min). f Total ROS generation for PS-CHO with ALDH (50 μg/mL) at different times (0–4 min) by DCFH assay (White light: 40 mW/cm2, 4 min). g The preparation of PS-CHO@ATRA-SS-ATRA. Created in BioRender. Jiajing, G. (2023) BioRender.com/u78h472. h The size distribution of PS-CHO@ATRA-SS-ATRA by DLS. i The morphology of PS-CHO@ATRA-SS-ATRA by TEM. Scale bar: 500 nm. j Cumulative PS-CHO release rate from PS-CHO@ATRA-SS-ATRA incubated with different concentration of GSH (0, 0.1, 1 and 10 mM) by HPLC. (n = 3 independent samples; means ± SD). All enzymatic reactions were performed in PBS solution (0.1 M, pH 7.4) containing NAD(H)+ (1 mM).

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