Fig. 4: In vitro differentiation-inducing ability of nano-prodrug. | Nature Communications

Fig. 4: In vitro differentiation-inducing ability of nano-prodrug.

From: Self-assembled aldehyde dehydrogenase-activatable nano-prodrug for cancer stem cell-enriched tumor detection and treatment

Fig. 4

a The images of 4T1 cells in tumor-sphere formation assay after different drug treatments, Scale bar: 500 μm. b The tumor-sphere formation numbers after different drug treatments (n = 3 independent biological samples; means ± SD). c The images of immunofluorescence for detecting the HIF-1α expression of 4T1 tumor-sphere cells after different drug treatments. Nuclei were stained by DAPI. Scale bar: 100 μm. d The proportions of ALDH+ cells in 4T1 tumor-sphere cells after different drug treatments measured by flow cytometry. e–g The stemness-related genes (Sox2, Nanog and Oct4) of 4T1 tumor-sphere cells after different treatments quantified by qPCR (n = 3 independent biological samples; means ± SD). L: white light (40 mW/cm2, 4 min). The statistical significance is analyzed by one-way ANOVA with a Tukey post hoc test, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Saline (G1), PS-CHO + L (G2), ATRA-SS-ATRA (G3), PS-CHO@ATRA-SS-ATRA (G4), PS-CHO@DSPE-PEG2000 + L (G5), and PS-CHO@ATRA-SS-ATRA + L (G6).

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