Fig. 1: ANI clustering showing genomovar and phylogroup structure for the Sal. ruber and E. coli genomes used in this study.

All vs. all ANI values were computed for Sal. ruber (A) and E. coli and relatives (E. fergusonii/Escherichia clades I–III) (B) using FastANI with default settings. Hierarchical clustering was performed with average linkage using Euclidean distances. Phylogroups were determined from a concatenated core gene tree for each species and with ClermonTyping (see figure key for details). Genomovar assignments were called based on ANI values (see figure key).