Fig. 1: Binding spectra of sterols with CYP5122A1 and CYP51. | Nature Communications

Fig. 1: Binding spectra of sterols with CYP5122A1 and CYP51.

From: CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles

Fig. 1

Purified recombinant CYP5122A1 (A) and CYP51 (B) (in the oxidized ferric form) were incubated with an increasing amount of sterol ligands and difference spectra were recorded over a reference sample that only contained protein and buffer. Dissociation constant (Kd) was derived by fitting the equation ΔA = ΔAmax[L]/(Kd + [L]) to the peak-to-trough absorbance difference (ΔA) versus the ligand concentration ([L]) curve, where ΔAmax is the maximal amplitude of the spectral response.

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