Fig. 3: 1D NMR spectra of the purified metabolite (unknown) of lanosterol from incubations with CYP5122A1. | Nature Communications

Fig. 3: 1D NMR spectra of the purified metabolite (unknown) of lanosterol from incubations with CYP5122A1.

From: CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles

Fig. 3

1H (A) and 13 C (B) NMR spectral comparison between lanosterol (reference) and the purified metabolite indicating the presence of the aldehyde group in the purified metabolite. Hashtags (#) indicate signals of an impurity present in both the lanosterol standard and the unknown sample (see Supplementary Fig. 7 and Supplementary Table 3 for chemical shifts and proposed partial structure of this impurity). Asterisks (*) indicate signals of grease contamination (broad peak at 1.26 ppm and the multiplet at 0.86 ppm).

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