Fig. 5: CYP5122A1 is indispensable during the amastigote stage. | Nature Communications

Fig. 5: CYP5122A1 is indispensable during the amastigote stage.

From: CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles

Fig. 5

BALB/c mice were infected (i.p.) with day 3 stationary phase promastigotes (5 ×108 cells/mouse) and treated with GCV or PBS as described in Methods. A Spleen weights from uninfected and infected mice were measured at 4- or 7-weeks post infection. Parasite numbers in infected spleens were determined by qPCR (B) and limiting dilution assay (C) at the indicated times. D The pXNG4-22A1 plasmid copy numbers in amastigotes were determined by qPCR (#/cell ± SDs). Bars and error bars represent the means and standard deviations from 3 to 12 independent repeats. Two-tailed t test without adjustment (*p < 0.05, **p < 0.01, ***p < 0.001). Source data are provided as a Source Data file.

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