Fig. 9: Effects of SARS-CoV-2 infection on mucociliary clearance.

a MUC5AC dot blot on apical rinsates from infected and uninfected cultures over time. DPI = days post-infection. N = 1 culture from each of 3 donors per time per condition. b Quantification of MUC5AC signal in a. Infected cultures in blue and mock in pink. Error bars are mean ± standard deviation. N = 1 culture from each of 3 donors per time per condition. c Projected image showing apoptotic nuclei tracks (NucView 530, magenta) moving over a focus of infection (GFP, green) over the course of 0.8 s shortly after mucus rinses at 120 HPI. Right panels are inverted greyscale single channels. Field of view is at the edge of a larger circular focus underlying a mucus disc. Arrowheads point to moving NV puncta; curved pink arrow shows the direction of rotation of the larger mucus disc. Scale bar = 200 µm. Representative image from one experiment with 3 replicates for each of two donors. d Temporal color code projection of the GFP channel of a whole infected culture with a mucus rinse at 120 hpi (one representative image). Scale bar = 1 mm. Representative image from one experiment with 3 replicates for each of two donors. e GFP+ spots at each timepoint for 120 hpi rinsed cultures (n = 3 replicates for two donors). The vertical dashed line marks the time of rinse. Lines are individual cultures colored by the donor. f First derivative of splines fitted to GFP+ spots count at each frame of videos summarized in (e). Spar = 0.1 for spline fitting. Lines are colored by the donor. Loess fit line with span = 0.05 shown in bold; the gray outline is the standard error. Source data for the blot and all plots are provided as a Source Data file.