Fig. 6: Natural IELs-derived TGF-β1 promote OVA-induced oral tolerance.
From: Dietary nucleic acids promote oral tolerance through innate sensing pathways in mice
a Immunoblot analysis of the expression of transgenic CRISPR-Cas9 in natural CD8αα+ IELs. b Immunoblot analysis of the expression of TGF-β1 and TGF-β3 in natural CD8αα+ IELs by sgRNA-targeted knock-down. c–e MS DKO mice were first transferred without or with sgEV, sgTgfb1, or sgTgfb3 natural CD8αα+ IELs followed by induction of OVA-mediated oral tolerance (n = 5), then DTH responses were measured (c), the effector cytokines of total spleen cells (d) or serum OVA-specific antibody levels (e) were analyzed. f–h C57BL/6 mice that were pre-fed PD for 6 weeks after their weaning, then transferred without or with sgEV, sgTgfb1, or sgTgfb3 natural CD8αα+ IELs in the last 2 weeks, and followed by induction of OVA-mediated oral tolerance (n = 5), then DTH responses were measured (f), the effector cytokines of total spleen cells (g) or serum OVA-specific antibody levels (h) were analyzed. Data are mean ± s.e.m. and from one experiment representative of two (a, b) or three (c–h) independent experiments. Statistics: two-tailed Student’s t test. Source data are provided as a Source Data file.
