Fig. 6: Phenotypes induced by FTO^rs9939609-A are reversed by FTO inhibition.

a Left: Western blot of phospho-AKT, phospho-FOXO and GAPDH protein abundance in FTO^rs9939609-TT-myotubes: H1, H7, H9 and FTO^rs9939609-A-myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2, treated with DMSO, 200 nM bisantrene or 50 μM entacapone for 7 days. Right: quantification of the abundance of insulin signaling proteins, n = 3 biologically independent samples. b Quantitative RT-PCR for myogenic markers in FTO^rs9939609-TT-myotubes and FTO^rs9939609-A-myotubes treated with DMSO, 200 nM bisantrene or 50 μM entacapone for 7 days, n = 3 biologically independent samples. c Quantification of fold change in FTO^rs9939609-TT-myoblasts and FTO^rs9939609-A-myoblasts numbers, treated with DMSO, 200 nM bisantrene or 50 μM entacapone for 7 days, n = 3 biologically independent samples. d Left: representative images of senescent FTO^rs9939609-TT-myoblasts and FTO^rs9939609-A-myoblasts, treated with DMSO, 200 nM bisantrene or 50 μM entacapone FTO inhibition for 7 days by passage 16. Senescent cells were stained for β-galactosidase (SA-βgal). Scale bars, 100 μm. Right: quantification of percentage of SA-βgal+ senescent FTO^rs9939609-TT-myoblasts and FTO^rs9939609A-myoblasts, n = 3 biologically independent samples. e Left: representative images of FoxO1-GFP+ nuclei numbers at the end of 14 days’ exposure to 1% HFD serum, treated with DMSO, 200 nM bisantrene or 50 μM entacapone. Scale bars, 200 μm. Right: quantification of the percentage of FoxO1-GFP+ nuclei numbers, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. P values were calculated by two-tailed unpaired t-test. *P < 0.05, **P < 0.01. Source data are provided as a Source Data file.