Fig. 4: Experimental analysis and theoretical calculation on antioxidase-mimetic activities.
a Schematic diagram of the cascaded catalysis of SOD-like and CAT-like reactions. Atomic color coding in the structure: Ru, yellow; Cu, blue; O in Ru-Cu/EDHJ, pink; O in substrates, red; and H, white. b SOD-like activity of different materials (if not specifically stated, EDHJ group refers to CeO2@Cu2O; n = 3 independent experiments, data are presented as mean ± SD). c Time-dependent CAT-like performances via TiSO4-based method with the presence of biocatalysts and H2O2 (n = 3 independent experiments, data are presented as mean ± SD). d H2O2 elimination ratio of different materials (n = 3 independent experiments, data are presented as mean ± SD). e The produced O2 concentration was measured by an oxygen dissolving meter with the presence of biocatalysts and H2O2. f Vmax, Km, and TON values of Ru-Cu/EDHJ and Ru-O/EDHJ. g Comparative analysis of the Vmax and TON with previously reported biocatalysts. h CAT-like pathways (atomic color coding in the structure: Ru, yellow; Cu, blue; O in Ru-Cu/EDHJ, pink; O in substrates, red; and H, white) and (i) Gibbs free energy diagram of Ru-Cu/EDHJ and Ru-O/EDHJ. j Calculated-pCOHP of O 2p orbital and Ru 4d orbital in O2* and (k) corresponding ICOHP values of Ru-O bonds. l Schematic illustration of d orbital energy levels varies with coordination configurations. Vmax is the maximal reaction velocity, Km is the Michaelis constant, TON is the turnover number. In (b, d), statistical significance was calculated using a two-tailed Student’s t-test; all tests were two-sided. In (j), a.u. indicates the arbitrary units. Source data are provided as a Source Data file.
