Fig. 5: In vitro ROS clearance and stem cell protection by Ru-Cu/EDHJ. | Nature Communications

Fig. 5: In vitro ROS clearance and stem cell protection by Ru-Cu/EDHJ.

From: Electron-donable heterojunctions with synergetic Ru-Cu pair sites for biocatalytic microenvironment modulations in inflammatory mandible defects

Fig. 5

a Fluorescence images, Ctrl (PBS), (b) flow cytometry, and (c) mean fluorescence intensity of DCFH-DA staining (n = 3 independent replicates), p(H2O2) < 0.0001, compared with Ctrl group; p(EDHJ-H2O2) = 0.0118, p(Ru-O/EDHJ-H2O2) = 0.0003, p(Ru-Cu/EDHJ-H2O2) < 0.0001, compared with H2O2 group. Scale bar: 100 μm. d Fluorescence images of 4-HNE (Green: F-actin, Red: 4-HNE, Blue: Dapi). Scale bar: 50 μm. e Quantitative analysis of mean fluorescence intensity of 4-HNE staining (n = 20 independent replicates), p(H2O2) = 0.0011, compared with Ctrl group; p(EDHJ-H2O2) = 0.0359, p(Ru-O/EDHJ-H2O2) = 0.0121, p(Ru-Cu/EDHJ-H2O2) =  0.0010, compared with H2O2 group. f Fluorescence images of paxillin (Green: F-actin, Red: paxillin, Blue: Dapi). Scale bar: 50 μm. g Quantitative analysis of the area of cells (n = 20 independent replicates), p(H2O2) < 0.0001, compared with Ctrl group; p(Ru-O/EDHJ-H2O2) = 0.0011, p(Ru-Cu/EDHJ-H2O2) < 0.0001, compared with H2O2 group. h Quantitative analysis of focal adhesion plaque of paxillin area (n = 3 independent replicates), p(H2O2) = 0.0144, compared with Ctrl group; p(Ru-Cu/EDHJ-H2O2) = 0.0050, compared with H2O2 group. i The dead cell ratio counted with the treatment of materials and H2O2 after live/dead staining (n = 5 independent replicates), p(H2O2) = 0.0001, compared with Ctrl group; p(Ru-O/EDHJ-H2O2) = 0.0003, p(Ru-Cu/EDHJ-H2O2) < 0.0001, compared with H2O2 group. j Schematic illustration of cell effects upon the addition of high-concentration H2O2 and Ru-Cu/EDHJ protection. Data are presented as means ± SD., *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001, ns, no significance; statistical significance was calculated using one-way ANOVA followed by Tukey’s post-hoc test for multiple comparisons, all tests were two-sided. In (c, e, h), a.u. indicates the arbitrary units. Experiments were repeated independently (a, b, d, f) three times with similar results. Source data are provided as a Source Data file.

Back to article page